The kit is designed for multiplex qPCR with up to five pairs of primers and five TaqMan probes.
In multiplex qPCR amplification, a DNA-dependent DNA polymerase amplifies DNA templates and its 5′-3′exonuclease cleaves a TaqMan probes to generate fluorescent signals.
The kit contains Taq-Probe DNA polymerase, which is specially engineered for TaqMan probes and its increased 5′-3′ exonuclease activity generating S-shaped curves.
