Enzyme for PCR

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  2X qPCR Master Mix

  The kit is designed for multiplex qPCR with up to five pairs of primers and five TaqMan probes.
  In multiplex qPCR amplification, a DNA-dependent DNA polymerase amplifies DNA templates and its 5′-3′exonuclease cleaves a TaqMan probes to generate fluorescent signals.
  The kit contains Taq-Probe DNA polymerase, which is specially engineered for TaqMan probes and its increased 5′-3′ exonuclease activity generating S-shaped curves.
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  2X RT-PCR Master Mix

  2X RT-PCR Master Mix is used for real-time qualitative and quantitative multiplex RT-PCR amplifications with TaqMan probes.
  The kit is designed for multiplex RT-PCR with up to five pairs of primers and five TaqMan probes.
  The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and RNA templates.
  In a single-tube format, multiplex RNA templates are first converted to cDNAs by a reverse transcriptase, then a DNA-dependent DNA polymerase amplifies the cDNAs and its 5′-3′exonuclease cleaves TaqMan probes to generate fluorescent signals.
  For the reverse transcription step, this kit uses a super-efficient Thermophilic Reverse Transcriptase (US patent pending), a thermophilic type A polymerase, which works best at 60-62°C, overcoming difficulties due to high GC content and abundant secondary structure. The RTase efficiently synthesizes a complementary DNA strands on RNA templates with 1-2 unit per 20μL of reaction. The RTase reversely-transcribes single digit copies of target RNA molecules consistently.
  The kit also contains qPCR polymerase, which is specially engineered for TaqMan probes and its increased 5’-3’ exonuclease activity generating S-shaped curves.
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  qPCR Polymerase

  This DNA-dependent DNA polymerase is specially engineered for real-time PCR and RT-PCR amplification with TaqMan probe, singleplex or multiplex.
  It is used together with Thermophilic Reverse Transcriptate (Cat. 20230301) for RT-PCR.
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  Thermophilic Reverse Transcriptase

  This reverse transcriptase or RNA-dependent DNA polymerase is used for real-time-fluorescent qualitative and quantitative RT-PCR amplifications with SYBR Green dye or TaqMan probe.
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  DNA Polymerase

  This DNA-dependent DNA polymerase is used real-time PCR amplification with SYBR Green dye, particularly for STR amplification.