Product Description
This reverse transcriptase or RNA-dependent DNA polymerase is used for real-time-fluorescent qualitative and quantitative RT-PCR amplifications with SYBR Green dye or TaqMan probe.
Characteristics
• The reverse transcriptase is a thermophilic type A polymerase with optimal temperatures at 60-62oC.
• The RTase is easily heat-inactivated at ≥90°C for 1min.
• The RTase efficiently synthesizes a complementary DNA strand on RNA template from a gene-specific primer, ≤1 unit per 20μL of singleplex reaction.
• The RTase reversely-transcribes single digit copies of target RNA molecules consistently.
• The preferable PCR product size is ≤150bp.
• It is particularly suitable to one-step real-time qualitative and quantitative RT-PCR.
Catalogue
|
NO. |
Item |
Product Name |
Specification |
Concentration |
|
1 |
Cat.20230227 |
DNA Polymerase |
2500U/Bottle |
25U/μL |
|
2 |
Cat.20230228 |
DNA Polymerase |
2500U/Bottle |
25U/μL |
|
3 |
Cat.20230215 |
5X STR Buffer |
5mL/Bottle |
- |
|
4 |
Cat.20230110 |
2X qPCR Master Mix |
1mL/Bottle |
- |
|
5 |
Cat.20230115 |
2X RT-PCR Master Mix |
1mL/Bottle |
- |
|
6 |
Cat.20230226 |
qPCR Polymerase |
2500U/Bottle |
25U/μL |
|
7 |
Cat.20230301 |
Thermophilic Reverse Transcriptase |
500U/Bottle |
10U/μL |
|
8 |
Cat.20230210 |
5X PCR Buffer |
5mL/Bottle |
- |